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prСД CEN/TS 17883:2023

Environmental characterization of leachates from waste and soil using reproductive and toxicological gene expression in Daphnia magna

General information

60.55     Nov 14, 2022

60.60   

BDS

ТК-15

Technical Specification

13.030.01     13.060.70     13.080.99  

Scope

This document specifies the crucial steps of a quantitative real-time polymerase chain reaction (qPCR) method to quantify the abundance of specific mRNA molecules extracted from Daphnia magna.
The method allows the identification of molecular responses to exposures for potentially toxic substances through the analysis of the abundance of specific mRNA molecules. In this document, the central genes involved in reproductive and toxic responses are included.
NOTE The selection of genes can be adapted to specific exposure conditions, for example, exposure to known toxic substances, by adding genes known to respond to a specific insult.
The present method allows for rapid, robust and sensitive detection of molecular responses and can be used to analyse the toxic effects of water leachates from soil and waste. The method gives information of the concentration of a substance or test-liquid at which toxic effects begin to occur prior to observations of reproductive or toxic effects at higher levels of organization, which reduces the need for the use of safety factors in toxicity assessment.
The method is useful in several types of risk assessment. In this document, the genes studied are appropriate for the assessment of the risks when recycling materials and for the classification of waste, but the method can be adapted to other types of risk assessment by including other genes.

Life cycle

NOW

IN DEVELOPMENT
prСД CEN/TS 17883:2023
60.55 Ratification completed (DOR)
Nov 14, 2022

Related project

Adopted from CEN/TS 17883:2022 IDENTICAL