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БДС EN ISO 11063:2020

Soil quality - Direct extraction of soil DNA (ISO 11063:2020)

Nov 17, 2020

General information

60.60     Nov 17, 2020

BDS

ТК-15

European Standard

13.080.30  

English  

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Scope

The present document specifies a method for direct extraction of DNA from soil samples to analyse the abundance and composition of microbial communities by various techniques of molecular biology including real-time quantitative PCR (qPCR). This method is mainly dedicated to agricultural and forest soils. This method can possibly not be suitable for soils rich in organic matter (e.g. peat soils) or soils heavily polluted with organic pollutants or heavy metals.
The direct extraction of DNA from soil samples provides unique insight into the α- and β-diversity of microbial communities. Next-generation sequencing of amplicons obtained by PCR (polymerase chain reaction) amplification of soil DNA constitutes a promising domain which will in the near future contribute to the development of routine tools to monitor microbial communities in soil environments.

Life cycle

PREVIOUSLY

WITHDRAWN
БДС EN ISO 11063:2013

NOW

PUBLISHED
БДС EN ISO 11063:2020
60.60 Standard published
Nov 17, 2020

Related project

Adopted from EN ISO 11063:2020

Adopted from ISO 11063:2020